This is a full-time, permanent position on Crick terms and conditions of employment.
Overview
The area of pluripotent stem cell research is a dynamic field with a broad scope of applications due to the ability of the cells to differentiate down lineages representative of all three germ layers. Apart from the potential clinical application of human pluripotent stem cells in the field of cell therapies, the use of these cells is well established in areas of assay development, modelling of developmental pathways and disease modelling. The utilisation of these applications coupled with the use of gene editing tools such as CRISPR makes this type of biological system ideal to study the effects of gene knockout or gene modification. The Human Embryo and Stem Cell Unit (HESCU) is a core facility and supports and develops workflows with Crick scientists that involve the use of human pluripotent stem cells. HESCU has a multidisciplinary user base that is dynamic, evolving and expanding and with this comes the opportunity to be involved in cutting edge research.
Project summary
HESCU provides and maintains a well-equipped laboratory space and as a communal lab space, facilitates interaction between the groups working with human pluripotent stem cells fostering collaboration, exchange of ideas and the sharing of protocols between the scientific groups who use the facility. We currently work with users to provide support and promote best practice, in the areas of growing pluripotent stem cells, differentiation, characterisation, banking and method development. In addition, we are involved in the reprogramming of somatic cells to produce pluripotent stem cell lines. We are looking to extend our portfolio to include gene editing technologies to add value to the core service.
We are looking for a motivated, collaborative, scientist to work with HESCU staff and the user base on established protocols and to help maintain and aid in the development of the portfolio of services offered by this core facility.
Key responsibilities
Provision and support for the day to day running of the facility, ensuring effective service delivery. These responsibilities include but are not limited to:
Housekeeping
Day to day management of stock
Cleaning laboratory spaces and equipment
Helping with operational activities and planned preventative maintenance
Provision of media and reagents
Inventory and stock control
Aliquoting reagents
Reagent preparation
Quality Control assisting with adopted methods
Immunofluorescence,
RT-PCR, Mycoplasma,
DNA and RNA extraction
Expansion of cell lines
Banking cell lines
Distribution of cell lines to users
Cryopreservation of cell lines
Thawing cell lines
Stability testing
Production of feeder cells
Key experience and competencies
The post holder should embody and demonstrate our core Crick values: bold, imaginative, open, dynamic and collegial, in addition to the following:
Essential
Qualifications, experience and competencies:
Degree in a biological subject
Experience in cell culture
Excellent communication and presentation skills.
Excellent organisational skills and record keeping with attention to detail
A good working knowledge of Microsoft Office and internet skills
Ability to prioritise workflows and deliver to deadlines
Highly self-motivated with a pro-active approach.
Proven ability to work both collaboratively and independently.
Accurate record keeping
Desirable
Qualifications, experience and competencies:
Experience in human pluripotent stem cell culture
Experience in a range of molecular biology techniques including RT-PCR and Real Time qRT-PCR.
Experience in Flow Cytometry and Immunofluorescence
Excellent inter-personal skills and a willingness to learn new skills
Ability to work with diverse databases
https://crick.wd3.myworkdayjobs.com/External/job/London/Laboratory-Research-Scientist---Human-Embryo-and-Stem-Cell-Unit_R433-1 36 1 Lyn Healy Stp |Human Embryo And Stem Cell Unit Team (Lyn Healy) Human Embryonic Stem Cell Eleanor.Cowan@crick.ac.uk R433 Search engines may index Yes Science 3 <p>Competitive with benefits, subject to skills and experience</p>
