Jonathan Stoye

Jonathan Stoye


Jonathan was born in Oxford but studied for a BA degree in Natural Sciences at the University of Cambridge (1970-73).

He then worked as a technician for five years in Basel, Switzerland before embarking on research supervised by Christoph Moroni leading to a PhD from the University of Basel.

Next, he joined John Coffin’s lab in Boston, USA spending seven years (1982-89) as a research associate before returning to the UK with an appointment at the MRC’s National Institute for Medical Research (NIMR). Since 2015 he has been a Senior Group Leader at the Francis Crick Institute. He was elected a Fellow of the Royal Society in 2017.

Qualifications and history

Friedrich Miescher Institute, Basel, Switzerland
Tufts University School of Medicine, Boston, USA
Research Associate
Medical Research Council National Institute for Medical Research, London, UK
Team Leader
Medical Research Council National Institute for Medical Research, London, UK
Head, Division of Virology
Francis Crick Institute
Senior group Leader

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Last updated : 09 December 2022 02:12

Laboratory Research Scientist - Human Embryo and Stem Cell Unit

Submitted by codeenigma-mas… on 20 September 2021

Reports to:

Principle Laboratory Research Scientist

Contact term:

This is a full-time, permanent position on Crick terms and conditions of employment.


The area of pluripotent stem cell research is a dynamic field with a broad scope of applications due to the ability of the cells to differentiate down lineages representative of all three germ layers. Apart from the potential clinical application of human pluripotent stem cells in the field of cell therapies, the use of these cells is well established in areas of assay development, modelling of developmental pathways and disease modelling. The utilisation of these applications coupled with the use of gene editing tools such as CRISPR makes this type of biological system ideal to study the effects of gene knockout or gene modification. The Human Embryo and Stem Cell Unit (HESCU) is a core facility and supports and develops workflows with Crick scientists that involve the use of human pluripotent stem cells. HESCU has a multidisciplinary user base that is dynamic, evolving and expanding and with this comes the opportunity to be involved in cutting edge research.

Project summary

HESCU provides and maintains a well-equipped laboratory space and as a communal lab space, facilitates interaction between the groups working with human pluripotent stem cells fostering collaboration, exchange of ideas and the sharing of protocols between the scientific groups who use the facility. We currently work with users to provide support and promote best practice, in the areas of growing pluripotent stem cells, differentiation, characterisation, banking and method development. In addition, we are involved in the reprogramming of somatic cells to produce pluripotent stem cell lines. We are looking to extend our portfolio to include gene editing technologies to add value to the core service.

We are looking for a motivated, collaborative, scientist to work with HESCU staff and the user base on established protocols and to help maintain and aid in the development of the portfolio of services offered by this core facility.

Key responsibilities

Provision and support for the day to day running of the facility, ensuring effective service delivery. These responsibilities include but are not limited to:


  • Day to day management of stock
  • Cleaning laboratory spaces and equipment
  • Helping with operational activities and planned preventative maintenance

Provision of media and reagents

  • Inventory and stock control
  • Aliquoting reagents
  • Reagent preparation

Quality Control assisting with adopted methods

  • Immunofluorescence,
  •  RT-PCR, Mycoplasma,
  •  DNA and RNA extraction

Expansion of cell lines

  • Banking cell lines
  • Distribution of cell lines to users
  • Cryopreservation of cell lines
  • Thawing cell lines
  • Stability testing
  • Production of feeder cells

Key experience and competencies

The post holder should embody and demonstrate our core Crick values: bold, imaginative, open, dynamic and collegial, in addition to the following:


Qualifications, experience and competencies:

  • Degree in a biological subject
  • Experience in cell culture  
  • Excellent communication and presentation skills.
  • Excellent organisational skills and record keeping with attention to detail
  • A good working knowledge of Microsoft Office and internet skills
  • Ability to prioritise workflows and deliver to deadlines
  • Highly self-motivated with a pro-active approach.
  • Proven ability to work both collaboratively and independently.
  • Accurate record keeping


Qualifications, experience and competencies:

  • Experience in human pluripotent stem cell culture
  • Experience in a range of molecular biology techniques including RT-PCR and Real Time qRT-PCR.
  • Experience in Flow Cytometry and Immunofluorescence
  • Excellent inter-personal skills and a willingness to learn new skills
  • Ability to work with diverse databases 36 1 Lyn Healy Stp |Human Embryo And Stem Cell Unit Team (Lyn Healy) Human Embryonic Stem Cell R433 Search engines may index Yes Science 3 <p>Competitive with benefits, subject to skills and experience</p>