Assessing cell size and cell cycle regulation in cells with altered TOR activity

Abstract

Target of rapamycin (TOR) regulates the growth of cells and organisms. Numerous growth-promoting and growth-arresting pathways converge on TOR; TOR acts as an important hub, balancing the pro- and anti-growth signals within a cell. Since it regulates growth at the cellular level, cell size can be used as an indirect readout of TOR activity. Here, we describe methods used to analyze cell size in cell culture and in the Drosophila wing.

Journal details

Volume 821
Pages 227-237
Publication date

Keywords

Type of publication