Benefits of chemical sugar modifications introduced by click chemistry for glycoproteomic analysesMore about Open Access at the Crick
Authors listBea Calle Ganka Bineva-Todd Andrea Marchesi Helen Flynn Mattia Ghirardello Omur Yilmaz Tastan Chloe Roustan Junwon Choi M Carmen Galan Ben Schumann Stacy A Malaker
Mucin-type O-glycosylation is among the most complex post-translational modifications. Despite mediating many physiological processes, O-glycosylation remains understudied compared to other modifications, simply because the right analytical tools are lacking. In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable. Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles. In doing so, we realized that the chemical modifications used in these studies have additional benefits that allow for improved analysis by tandem mass spectrometry. In this work, we built on this discovery by generating a series of new GalNAc analogue glycopeptides. We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal resporter reagents. Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.
Issue number 9
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Publisher website (DOI) 10.1021/jasms.1c00084
Europe PubMed Central 33871988