MST3 kinase phosphorylates TAO1/2 to enable Myosin Va function in promoting spine synapse development
More about Open Access at the CrickAuthors list
Sila Ultanir Smita Yadav Nicholas T Hertz Juan A Oses-Prieto Suzanne Claxton Alma L Burlingame Kevan M Shokat Lily Y Jan Yuh-Nung JanAbstract
Summary Mammalian Sterile 20 (Ste20)-like kinase 3 (MST3) is a ubiquitously expressed kinase capable of enhancing axon outgrowth. Whether and how MST3 kinase signaling might regulate development of dendritic filopodia and spine synapses is unknown. Through shRNA-mediated depletion of MST3 and kinase-dead MST3 expression in developing hippocampal cultures, we found that MST3 is necessary for proper filopodia, dendritic spine, and excitatory synapse development. Knockdown of MST3 in layer 2/3 pyramidal neurons via in utero electroporation also reduced spine density in vivo. Using chemical genetics, we discovered thirteen candidate MST3 substrates and identified the phosphorylation sites. Among the identified MST3 substrates, TAO kinases regulate dendritic filopodia and spine development, similar to MST3. Furthermore, using stable isotope labeling by amino acids in culture (SILAC), we show that phosphorylated TAO1/2 associates with Myosin Va and is necessary for its dendritic localization, thus revealing a mechanism for excitatory synapse development in the mammalian CNS.
Full text links
Publisher website (DOI) 10.1016/j.neuron.2014.10.025
Europe PubMed Central 25456499
Pubmed 25456499
Keywords
Type of publication