Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissueMore about Open Access at the Crick
Authors listYuan Zhao Mohamed Uduman Jacqueline HY Siu Thomas J Tull Jeremy D Sanderson Yu-Chang Bryan Wu Julian Q Zhou Nedyalko Petrov Richard Ellis Katrina Todd Konstantia-Maria Chavele William Guesdon Anna Vossenkamper Wayel Jassem David P D'Cruz David J Fear Susan John Dagmar Scheel-Toellner Claire Hopkins Estefania Moreno Natalie L Woodman Francesca Ciccarelli Susanne Heck Steven H Kleinstein Mats Bemark Jo Spencer
Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27CD45RB population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organised gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC, where somatic mutations are acquired.