Tissue clearing to examine tumour complexity in three dimensions
Abstract
The visualization of whole organs and organisms through tissue clearing and fluorescence volumetric imaging has revolutionized the way we look at biological samples. Its application to solid tumours is changing our perception of tumour architecture, revealing signalling networks and cell interactions critical in tumour progression, and provides a powerful new strategy for cancer diagnostics. This Review introduces the latest advances in tissue clearing and three-dimensional imaging, examines the challenges in clearing epithelia - the tissue of origin of most malignancies - and discusses the insights that tissue clearing has brought to cancer research, as well as the prospective applications to experimental and clinical oncology.
Journal details
Journal Nature Reviews Cancer
Volume 21
Issue number 11
Pages 718-730
Available online
Publication date
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Publisher website (DOI) 10.1038/s41568-021-00382-w
Europe PubMed Central 34331034
Pubmed 34331034
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